1 Intro

This report provides some basic description of the peptide identificaiton from database search. Users can use this to quickly check the overal quality of the experiment Users can download the clean peptide quantification matrix for downstream analysis

2 Take-home figures

  • Number of contaminant: 27

  • Number of reversed: 52

  • Number of qualified peptides: 16313

  • Number of quailfied peptide without intensity information(0 intensities): 695

  • Number of experiment: 24

  • All experiments: Xu_MLIspin_test_B6, Xu_MLIspin_test_B7, Xu_MLIspin_test_B8, Xu_MLIspin_test_B9, Xu_MLIspin_test_C6, Xu_MLIspin_test_C7, Xu_MLIspin_test_C8, Xu_MLIspin_test_C9, Xu_MLIspin_test_D6, Xu_MLIspin_test_D7, Xu_MLIspin_test_D8, Xu_MLIspin_test_D9, Xu_MLIspin_test_E6, Xu_MLIspin_test_E7, Xu_MLIspin_test_E8, Xu_MLIspin_test_E9, Xu_MLIspin_test_F6, Xu_MLIspin_test_F7, Xu_MLIspin_test_F8, Xu_MLIspin_test_F9, Xu_MLIspin_test_G6, Xu_MLIspin_test_G7, Xu_MLIspin_test_G8, Xu_MLIspin_test_G9

  • No meta information provided

3 Peptide Charge States

4 Peptide Length

5 Peptide Score distribution

6 Peptide Intensity distribution

In most cases, label-free quantification provides a decent way for metaproteomics profiling.

6.1 Sparisty Profile

Presence of valid intensity across samples

6.2 Sparsity cummulative curve

Figure shows how many peptides have more than N presence, which helps to set the presence cutoff

7 Intensity disitribution acrross samples/experiments

8 Overall Expression Profile

8.1 Heatmap

Heatmap of Presence 100

8.2 PCA analysis

9 Download the neat table

The table is clean peptides expression table, with reversed and contaminant removed. The values are the LFQ(with label free quantification turned on) or raw protein intensity from Maxquant output. You can download/export and start from this table for downstream analysis using the (i)Metalab faminly apps.

You can play with this table with our shiny appsshiny.imetalab.ca